Concentration Analysis

Background

Evaluating the concentration of samples containing nano-sized particles is a key parameter for researchers in many fields. Izon's qNano and qViro instruments provide:

• A Rapid, accurate, repeatable and cost-effective solution for concentration analysis
• Discriminating information at the individual particle level
• Coverage of a wide range of particle types & sizes

For researchers involved with the synthesis of all types of synthetic nanoparticles, evaluation of a range of biological samples, as well as those working in virology, gene therapy and vaccinology, who require an understanding of viral titre, this capability, combined with a concentration measurement range spanning seven orders of magnitude, provides a powerful concentration analysis tool.

Utilising Izon's qNano & qViro in conjunction with the pressure control capability provided by Izon's VPM (variable pressure module) provides an effective approach for measurement of sample concentration.

This methodology can be applied to all particle types, including both charged and uncharged particles, in a wide variety of electrolytes, across a wide range of sample concentration values.

Concentration Analysis Methodology

The relationship between an applied pressure and the measured frequency of blockade events is linear for a given particle set (see figure at left). The exact linear response (gradient) varies depending upon the concentration of the particles being measured.

For a given applied pressure (x) the blockade frequency response for two different samples are related by the relative gradient values (m) of the response lines. As the particle flux is proportional to concentration this allows the concentrations of the two samples to be directly related through their measured blockade frequency and gradient response over pressure.

Measurement of a standard at a single applied pressure setting provides a reference against which any other sample can be compared to obtain an accurate absolute concentration value.